Fig. 5: Elucidation of gltS as a drug potentiation target in P. aeruginosa. | Communications Biology

Fig. 5: Elucidation of gltS as a drug potentiation target in P. aeruginosa.

From: Systems-level exploitation of OxyR regulon unravels a potential antibacterial target in Pseudomonas aeruginosa

Fig. 5

A ROS generation in gltS, PAO1, and its derivatives. The median of fluorescence intensity of 50,000 log phase grown cells treated with 20 mM H2DCFDA is given. The assays were performed three times with independent cultures. Data are means ± SEM. Significance was determined by Student’s t test; p < 0.01; n = 3. Evaluation of susceptibility to killing by antibiotics. Time course survival analysis of gltS and PAO1 treated with gentamicin (B), tobramycin (C) and ampicillin (D). Error bars are indicated, mean ± SEM for all plots. Student’s t test; p < 0.01; n = 3. (E) Synteny analysis of GltS in ESKAPE pathogens. Complex syntenies were obtained by using P. aeruginosa GltS protein sequence as query sequence to search against indicated Pseudomonads species. A consistent color coding permitted the correct identification of both orthologs and paralogs. The genes corresponding to the query proteins are drawn in bold and boxed with light olive color.

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