Fig. 3: Reducing glucose concentration promotes mitochondrial translocation of PKM2.
From: Succinylation regulates boar sperm linear motility via reprogramming glucose metabolism
a, b After 3 h of incubation in HG or LG extenders, boar sperm were subjected to immunofluorescence analysis to evaluate subcellular localization. Mitochondria were identified with Mito Tracker™ Deep Red FM, nuclei were stained with Hoechst 33342. Immunofluorescence analysis of PKM2 in boar sperm. Bar = 10 μm (a). Quantitative data for immunofluorescence staining (b). Data are expressed as mean ± SD (n = 3). c, d After 3 h of incubation in HG or LG extenders, mitochondrial fractions were isolated from boar sperm for Western blot analysis. Western blotting of PKM2 in boar sperm mitochondria. Protein loading was normalized using an NDUFA7, and mitochondrial purity was validated with ODF2 (c), and Western blot band intensities were quantified with ImageJ software (d). Values represent mean ± SD (n = 3). a, b: Significant differences (p < 0.05) between treatments. FS fresh semen, HG high glucose, LG low glucose, RES resveratrol, SA succinic acid.
