Fig. 2: Dose-response analysis and determination of IC50 values of the selected lead compounds.
From: Exploiting the angiotensin-converting enzyme pathway to augment endogenous opioid signaling
A Chemical structures of the identified LOPAC hits—captopril (positive control), thiorphan, D609, and raloxifene hydrochloride. B Dose-response curves of the compounds obtained by fluorescence-based ACE enzymatic assay with error bars displaying SEM. The calculated IC50 values of the lead compounds from the fluorescence and mass spectrometry-based methods are provided in Table 1. Source data are provided in Supplementary Data 1.
