Fig. 6: Assessment of the antinociceptive effect of the lead compounds by acetic acid-induced writhing test.
From: Exploiting the angiotensin-converting enzyme pathway to augment endogenous opioid signaling
Eight-week old male ICR-CD1 mice (n = 4–6 animals/group) were injected with the lead compounds by i.c.v. (A, C) or i.p. (B, D) route 5 min prior to i.p. injection of 1% acetic acid according to the designed treatment regimen and number of writhings induced by acetic acid was recorded. Bar graphs display the writhings in various treatment groups: A captopril (100 µg/animal, i.c.v.), B captopril (50 mg/kg, i.p.), C raloxifene (100 µg/animal, i.c.v.), D raloxifene (108 mg/kg, i.p.). Data are shown as the mean ± SEM, with error bars displaying SEM. One-way ANOVA followed by Sidak’s post hoc multiple comparison test was used to determine the statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Source data are provided in Supplementary Data 1.
