Fig. 5: Censoring technology for use in mapping sets of hemi-brain BARseq sections from two separate mice to one another.
a Shows each set of hemi-brain sections mapped to CCFv3 for comparison of scope of tissue capture. b Shows initial alignment of hemi-brain tissue sections. c Shows alignment of tissue sections following estimation of diffeomorphism with censoring to take atlas hemi-brain to target hemi-brain. d Shows single target slice with cell type denoted by color. e, f Show intersecting atlas slice e before and f after alignment to target hemi-brain sections, with white arrows indicating regions of hippocampal (Subiculum in yellow, CA3 in orange) alignment post deformation. g, h Show the fraction of 100 μm3 cubes across the domain of the selected target sections with matching predominant cell type between atlas and target hemi-brain g globally across the whole section and h layer-specific. Total number of cubes per layer given in Supplementary Table 4. Predominant cell type per cube is shown for i, l target sections, j, m intersecting atlas section as initially aligned and k, n after diffeomorphic alignment.
