Fig. 2: Ribosome complex profiling (RCP-seq) captures small ribosomal subunits (SSU) and elongating ribosomes (80S) from the mouse cortical tissue.

a, b Content plots displaying the mapping of SSU, 80S, and total mRNA counts to the 5′ leaders, CDS, and 3′ trailers of the transcripts (n = 3). a shows the % count for each library and b displays the normalised counts to the total RNA. Footprint length distribution for the 5′ end of SSU fragments in the cortex for (c) translation initiation site (TIS) and (d) translation termination site (TTS). Footprint length distribution for the 5′ end of 80S fragments in the cortex for (e) translation initiation site (TIS) and (f) translation termination site (TTS). FracPos indicates the fraction of counts per position per gene.