Fig. 2: scRNA-seq reveals that astrocyte is most sensitive cell type to ferroptosis following Pb exposure. | Communications Biology

Fig. 2: scRNA-seq reveals that astrocyte is most sensitive cell type to ferroptosis following Pb exposure.

From: Lead binds HIF-1α contributing to depression-like behaviour through modulating mitochondria-associated astrocyte ferroptosis

Fig. 2

A Flow chart of scRNA-seq in mice brain tissue. Mice are allocated into Control and MPb groups. After 8 weeks of Pb exposure, the brain tissue of each group is extracted (n = 3). scRNA-seq is conducted on the single cells using 10×Genomics; B The UMAP plot demonstrating the annotation and colour codes for different cell types in Control and MPb groups (n = 3); C The representative marker genes of different cell types (n = 3). Dot size represents the proportion of cells expressing the gene, whereas the colour intensity represents the average expression; D The proportion of all cell types in Control and MPb groups; E The proportion of all cell types in Control and MPb groups (n = 3); F The ferroptosis score of the cell types in different groups by AddModuleScore; G The enrichment score of ferroptosis in different cell types by GSEA. H Immunofluorescence assays are conducted to detect the GFAP+ and PTGS2+ astrocytes in the Pb-exposed and control groups (n = 3). *P < 0.05 vs Control; **P < 0.01 vs Control. Comparisons between the two groups are made using Student’s t-test.

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