Fig. 9: Vdac1 knockdown rescued Pb-induced ferroptosis in astrocyte. | Communications Biology

Fig. 9: Vdac1 knockdown rescued Pb-induced ferroptosis in astrocyte.

From: Lead binds HIF-1α contributing to depression-like behaviour through modulating mitochondria-associated astrocyte ferroptosis

Fig. 9

A Fe2+ visualised by FerroOrange staining in Pb-exposed astrocytes infected with si-NC and si-Vdac1; B Fluorescence intensity of FerroOrange in Pb-exposed astrocytes infected with si-NC and Vdac1 analysed by ImageJ (n = 3); C Content of Fe2+ in Pb-exposed astrocytes infected with si-NC and si-Vdac1 (n = 6); D GSH, E MDA content in Pb-exposed astrocytes infected with si-NC and si-Vdac1 (n = 6); F Fluorescence peak of LipROS by C11-BODIPY; G The fluorescence intensity of C11-BODIPY in Pb-exposed astrocytes infected with si-NC and si-Vdac1 (n = 6); H Ultrastructure of mitochondria in astrocytes of different groups was imaged by transmission electron microscopy. Red arrowheads: shrunken mitochondria; I Gel electrophoresis plot and semi-quantification of GPX4 and PTGS2 in Pb-exposed astrocytes infected with si-NC and si-Vdac1; *P < 0.05 vs indicated group; **P < 0.01 vs indicated group; ***P < 0.001 vs indicated group. Comparisons are made with ANOVA, followed by Tukey’s multiple comparison tests.

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