Fig. 2: Structure of Cir bound by the MccV receptor binding domain.
From: Structural insights into Cir-mediated killing by the antimicrobial protein Microcin V
A Cryo-EM structure of the Cir/MccV RBD complex. The β-barrel and extracellular loops of Cir are shown as green ribbon with the plug domain highlighted in purple. The MccV RBD is shown in gold. The structure is inset into the cryo-EM density map with the NAPol micelle subtracted. OM outer membrane. B Cir/MccV RBD cryo-EM map colored to match model (contour level 0.120). C Illustration of the Cir/MccV binding pocket shown as cartoon (top) and as MccV surface (bottom). MccV interacts with the Cir extracellular loops in an area flanked by loops 2, 7, and 8 at the top and the plug domain below. This map and accompanying coordinates have been deposited to EMDB and PDB under codes EMD-49565 and 9NN6, respectively.
