Fig. 6: Mutation of the Cir TonB box inhibits MccV killing, not binding.
From: Structural insights into Cir-mediated killing by the antimicrobial protein Microcin V
A A zoom-in of the TonB box within the N-Terminal domain of Cir, highlighted in orange and with the mutated residues labeled. B Zone of inhibition assays using WT MccV secreted onto a lawn of WT or M33C/V35P Cir-expressing cells. We observed a zone of inhibition only when WT Cir was produced. C Comparison table for affinity and sensitivity for WT and M33C/V35P Cir. D BLI assay using WT and M33C/V35P Cir on WT MccV. 100 nM MccV was loaded to BLI sensors and they were subsequently probed with dilutions of M33C/V35P Cir, with 5 μM WT Cir included as a positive control and buffer only as a negative. Trace is representative of n = 4 experiments from two independent protein preparations.
