Table 1 Cryo-EM data collection and structure statistics
From: Structural insights into Cir-mediated killing by the antimicrobial protein Microcin V
Cir/MccV | |
|---|---|
Data collection | |
Magnification | 105,000× |
Voltage (keV) | 300 |
Total Dose (e/Å2) | 59.65 |
Number of frames | 30 |
Exposure time (s/frame) | 0.075 |
Defocus range (μm) | −0.8 to −2.4 |
Pixel size (Å) | 0.83 |
Image processing | |
Movies collected | 7606 |
Micrographs selected | 5960 |
Particles extracted | 7,726,727 |
Final map particles | 102,419 |
Symmetry imposed | C1 |
FSC threshold | 0.143 |
Final map resolution (Å) | 2.9 |
Resolution range (Å) | 3.06–2.71 |
Atomic model | |
Protein residues | 664 |
Chains | 2 |
Validation | |
Ramachandran favored (%) | 97.73 |
Ramachandran allowed (%) | 2.27 |
Ramachandran outliers (%) | 0 |
Rotamer outliers (%) | 0 |
RMSD bond lengths (Å) (# > 4σ) | 0.002 (0) |
RMSD bond angles (°) (# > 4σ) | 0.419 (0) |
Clash score | 5.60 |
Map CC (mask) | 0.87 |
Map CC (volume) | 0.88 |
Deposition IDs | |
PBD | 9NN6 |
EMDB | EMD-49565 |
