Fig. 5: The FtsZ levels in the gPCEM strain vary under different light conditions.

A Western blotting of FtsZ levels in the WT and the gPCEM strain cultured in BG11 medium with 1 mM of theophylline (TP) and 0.3 μM Cu²⁺ at different days and light conditions. Similar amounts of total proteins extracted from different strains were loaded on the gel, stained with Coomassie Brilliant Blue (middle, CBB), or probed with a polyclonal antibody against FtsZ (Bottom). Representative microscopic images of the indicated strains under different conditions are presented. LL: low light; SL: standard light; HL: high light. B FtsZ levels of the Δsuc-gPCEM strain in comparison with those of the WT and the gPCEM strain at the indicated days and light conditions as in (A). D0-D6: day 0 to day 6. C The transcription levels of the ftsZ gene in the WT, the gPCEM strain, and the Δsuc-gPCEM strain at the indicated time points and light conditions. The experiment was done with three technical replicates in three biological replicates. The Student’s t-test examined the differences between the WT (normalized to 1) and the other strains under the same conditions. Results were considered significant at ***p < 0.001, *p < 0.05. ns: no significance.