Fig. 1: Cellular and structural quality control of brain organoids.
From: Deciphering brain organoid heterogeneity by identifying key quality determinants
A Experimental paradigm depicting undirected differentiation of 12 hPSC lines until day 30 of brain organoid, followed by standard quality control. B Immunofluorescence (IF) images of organoids stained at day 30 with DAPI (blue), for SOX2 (green), and MAP2 (red). Cropped regions below, highlighted with white arrows. White scale bar: 500 µm. C Barplot depicting the number of VLS per section. Each dot represents the amount of VLS per section of one organoid slice. The color scale depicts the increase in VLS, ranging from green (high amount) to pink (low mount). Data represented as mean ± SD (n = 6 organoids). D Barplot depicting the MAP2+ area ratio to whole organoid slice. Each dot represents one organoid. Data represented as mean ± SD (n = 6 organoids). E Barplot depicting the percentage of PAX6+ individual organoids assessed by flow cytometry. Data represented as mean ± SD. F Representative brightfield images of high and low quality rated organoids at day 30 of differentiation of the lines HuES6, TMOi001A, CVB, and KOLF2.1J. Scale bar: 1 mm.
