Fig. 4: Experimental validation illustrating that mesenchymal cells are associated with higher organoid Feret diameter in sections.
From: Deciphering brain organoid heterogeneity by identifying key quality determinants
A Representative images of organoids from four hPSC lines. Stained with CD73 (blue), CD105 (yellow), MAP2 (magenta), and DAPI. Scale bar 500 µm. B Scatter plot depicting mean fluorescent intensity of CD105 (x-axis) and CD73 (y-axis). Individual dots represent individual organoids (n = 65). Color depicts 12 hPSC lines (legend on right). Significant positive correlation (Spearman r = 0.7, p = 1.08 × 10−10) is depicted in the graph. C Scatter plot depicting the organoid section’s Feret diameter (x-axis) and mean fluorescent intensity of CD105 (y-axis). Individual dots represent individual organoids (n = 65). Color depicts 12 hPSC lines (legend on right). Significant positive correlation (Spearman r = 0.44, p = 0.00029) is depicted in the graph. D Scatter plot depicting the organoid section’s Feret diameter (x-axis) and mean fluorescent intensity of CD73 (y-axis). Individual dots represent individual organoids (n = 65). Color depicts 12 hPSC lines (legend on right). Significant positive correlation (Spearman r = 0.39, p = 0.00146) is depicted in the graph.
