Fig. 1: Arc and c-Fos are differentially expressed across numerous quantified brain regions. | Communications Biology

Fig. 1: Arc and c-Fos are differentially expressed across numerous quantified brain regions.

From: Immediate-early genes Arc and c-Fos show divergent brain-wide expression following contextual fear conditioning

Fig. 1

A Experimental timeline and conditions. Red indicates transient expression of IEGs Arc and c-Fos following behavior-driven neuronal activity. B Percentage of time spent freezing was greater in 1-day and 5-day retrieval groups compared to no shock and encoding groups. C Left: representative coronal hemisphere section showing Arc (green) and c-Fos (magenta) expression with a Hoechst nuclear counterstain (blue). Right: schematic coronal hemisphere section showing quantified brain regions (purple), adapted from the Allen Mouse Brain Atlas (Mouse, P56) at http://atlas.brain-map.org83. D Expression levels of Arc (green) and c-Fos (magenta) as measured by percentage of all cells per region expressing each marker. Group means are represented by the thick inner line; SEM are represented by thin outer lines. N = 4 (no shock) or 8 (encoding, 1-day retrieval, 5-day retrieval) mice. Error bars indicate ±SEM. ****p < 0.0001. DG dentate gyrus, BLA basolateral amygdala complex, RSCv ventral retrosplenial cortex, PVT paraventricular nucleus of the thalamus, LH lateral habenula. A was created in BioRender. Bulthuis, N. (2025) https://BioRender.com/ih5c2sa.

Back to article page