Fig. 5: SiPc is inducing envelope stress in E. coli K-12 MG1655. | Communications Biology

Fig. 5: SiPc is inducing envelope stress in E. coli K-12 MG1655.

From: Photosensitizer-specific bacterial stress responses in Escherichia coli reveal distinct targets in photoinduced inactivation

Fig. 5

SiPc without photo activation (SiPc, dark). Shown are growth curves of the reporter strain (A), final optical densities (B), YFP/CFP expression of the Pspy module over time (C), and total YFP expression (D) in response to the indicated concentration of SiPc. At 5 µM, the Pspy module shows a mild response (C) that is associated with reduced growth rate (A) and a reduced final optical density at the end of the experiment (B). SiPc with photo activation (SiPc, light). Shown are growth curves of the reporter strain (E), final optical densities (F), YFP/CFP expression of the Pspy module over time (G), and total YFP/CFP expression (H) in response to the indicated concentration of SiPc after photo activation. Light activation of SiPc results in an immediate stop in the increase of OD at 5 µM SiPc (E), and the response of the Pspy module to 1 µM SiPc is stronger than to 0.1 and to 5 µM SiPc, at which the response is actually reduced when compared to the control (G, H). The dashed lines indicate the time point of the addition of the test substance. Shown are the results of representative experiments. The columns (D, H) represent the average results of three biological replicates (*P < 0.05; **P < 0.01; ***P < 0.001).

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