Fig. 2: Loss of TORC2 function promotes heterochromatin formation in the rDNA region.

ChIP-qPCR data showing IP/input ratios for H3K9me2 (a), H3K9me3 (b), and total H3 (c) in WT and the tor1∆ mutant. Bars represent means ± SEM, n = 3 experiments. d Images showing GFP-Swi6 (green) and Gar2-mCherry (red) in WT and the tor1∆ mutant. Scale bar = 5 µm. e Schematic diagram illustrating the positional relationship between GFP-Swi6 dots and Gar2-mCherry. GFP-Swi6 located at the edge and inside of the nucleolus was defined as localized to rDNA. Number of GFP-Swi6 dots located at the nucleolar edge (f) and inside the nucleolus (g) in WT and the tor1∆ mutant. Data represent the mean ± SEM of three independent experiments (WT replicate cell counts: 58, 72, and 68; tor1∆: 67, 70, 83). The percentage of cells with ≥ 2 internal GFP-Swi6 foci was significantly higher in tor1∆ (23.2%) than in WT (10.6%). Statistical significance was determined by two-sided Fisher’s exact test (p = 0.0007). h ChIP-qPCR showing IP/input ratios for GFP-Swi6 at each point of rDNA in WT and the tor1∆ mutant. Bars represent means ± SEM, n = 3 experiments. p-values were calculated by Student’s t-test. ***p < 0.001, **p < 0.01, *p < 0.05.