Fig. 3: Loss of Gad8 function induces heterochromatin formation in the rDNA region and suppresses rRNA transcription. | Communications Biology

Fig. 3: Loss of Gad8 function induces heterochromatin formation in the rDNA region and suppresses rRNA transcription.

From: TORC2 inactivation promotes heterochromatin formation in rDNA and prolongs viability of quiescent fission yeast cells

Fig. 3

a ChIP-qPCR data showing the IP/input of Gad8-FLAG in the rDNA region under glucose-rich (dark pink) and glucose-poor (light pink) conditions. Endogenous Gad8 without an epitope tag served as the negative control (gray). Bars represent means ± SEM, n = 3 experiments. Immunoblot analysis of Gad8-FLAG and α-tubulin protein levels under glucose-rich and -poor conditions (b) and in WT and tor1∆ cells (c). d ChIP-qPCR analysis showing fold enrichment of Gad8-FLAG in the rDNA region relative to the act1 gene in WT and the tor1∆ mutant. Bars represent means ± SEM, n = 3 experiments. ChIP-qPCR showing IP/input ratios for H3K9me2 (e) and H3K9me3 (f) in the rDNA region in WT and the gad8∆ mutant. Bars represent means ± SEM, n = 3 experiments. g Relative amounts of rRNA to act1 mRNA in WT and the gad8∆ mutant. Bars represent means ± SD, n = 3 experiments. p-values were calculated by Student’s t-test. ***p < 0.001, **p < 0.01, *p < 0.05.

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