Fig. 4: Dissociation of Paf1C from rDNA in coordination with Gad8 triggers heterochromatin formation. | Communications Biology

Fig. 4: Dissociation of Paf1C from rDNA in coordination with Gad8 triggers heterochromatin formation.

From: TORC2 inactivation promotes heterochromatin formation in rDNA and prolongs viability of quiescent fission yeast cells

Fig. 4

a Comparison of Leo1-FLAG enrichment in the rDNA region relative to act1 gene under glucose-rich (dark pink) and glucose-poor (light pink) conditions. Bars represent means ± SEM, n = 3 experiments. b ChIP-qPCR data showing Leo1-FLAG enrichment in the rDNA region relative to the act1 gene in WT and the gad8∆ mutant. Bars represent means ± SEM, n = 3 experiments. Enrichment levels of H3K9me2 (c) and H3K9me3 (d) shown as IP/input ratios at the 18S, 5.8S, and 28S regions in WT and the leo1∆ mutant. Bars represent means ± SEM, n = 3 experiments. e Quantification of total rRNA levels normalized to act1 mRNA in WT and the leo1∆ mutant. Bars represent means ± SD, n = 3 experiments. p-values were calculated by Student’s t-test. ***p < 0.001, **p < 0.01, *p < 0.05.

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