Fig. 5: Inactivation of both TOR pathways is associated with prolonged viability of quiescent cells.

Relative levels of H3K9me2 (a) and H3K9me3 (b) compared to the pericentromeric region (dg) in WT and the tor1∆ mutant at 18S, 5.8S, and 28S rDNA loci, with ( + rapa) or without (-rapa) rapamycin treatment. Bars represent means ± SEM, n = 3 experiments. c rRNA abundance normalized to act1 mRNA in WT and the tor1∆ mutant with ( + rapa) or without (-rapa) rapamycin treatment. Bars represent means ± SD, n = 3 experiments. d Schematic diagram illustrating the method for measuring the viability of quiescent cells. Cells were cultured for 2 days to induce a nondividing state, followed by continuous culturing for an additional 5 days. Equal volumes of cell culture were spread onto agar plates, and the number of viable cells was counted. Viability on Day 0 was used as the baseline (100%). e Graph showing the cell viability of WT and the tor1∆ cells with ( + rapa) or without (-rapa) rapamycin treatment. Data are presented as mean ± SD, n = 3 biological replicates. f Graph showing the cell viability of tor1∆ and tor1∆ clr4∆ cells with ( + rapa) or without (-rapa) rapamycin treatment. Data are presented as mean ± SD, n = 3 biological replicates. p-values were calculated by Student’s t-test. ***p < 0.001, **p < 0.01, *p < 0.05.