Fig. 4: Effects of unilateral activation/inactivation of CaMKIIa-RNs on the body orientation during locomotion.

A–C Silhouettes of the top views of the walking mouse at the moments of the maximal displacement of the spine toward the ipsilateral hindlimb [at the moment of the contralateral hindlimb toe off (Contra-TO), left panels] and toward the contralateral limb [at the moment of the ipsilateral hindlimb toe off (Ipsi-TO), right panels] in control (A) and during activation (B) and inactivation (C) of the right CaMKIIa-RNs. White dots are markers on the spine. The red dot indicates the point on the spine that exhibits maximal left-right oscillations during locomotion. The black scale indicates the body width with its middle considered as ”0” and the ipsilateral and contralateral edges of the body as +100% and –100%, respectively. D, E Values of the spine position (a displacement of the spine from the left-right body edges midline; D) and abduction/adduction asymmetry index (I_Ab/ad; E) during locomotion in individual animals, as well as the corresponding mean ± SD values, before (Control) and during unilateral activation and inactivation of CaMKIIa-RNs. F,G Values of the ratio between locomotor parameters (stance durations, swing durations, and duty cycles) of the contralateral and ipsilateral hindlimbs in individual animals, as well as the corresponding mean ± SD values, before (Control) and during unilateral activation and inactivation of CaMKIIa-RNs. In D, E: N = 5, n = 40 for control and n = 43 for unilateral activation; N = 4, n = 47 for control and n = 41 for unilateral inactivation. In F, G: N = 4, n = 39 for unilateral activation and N = 4, n = 42 for unilateral inactivation. In D, E Abbreviations as in Fig. 2.