Fig. 9: Neurotransmitter phenotypes of CaMKIIa-RNs located in MdV-IRt area.
From: Role of CaMKIIa reticular neurons of caudal medulla in control of posture
A, B Two representative images with GFP expression in CaMKIIa-RNs (green cells in the upper panels), with in situ hybridization for Vglut2 mRNA identifying glutamatergic RNs (A, red cells in the middle panel), with in situ hybridization for Vgat mRNA identifying inhibitory RNs (B, red cells in the middle panel), with co-expression of GFP and for Vglut2 mRNA identifying glutamatergic CaMKIIa-RNs (A, yellow cells in the lower panel), and with co-expression of GFP and for Vgat mRNA identifying inhibitory CaMKIIa-RNs (B, yellow cells in the lower panel). White arrowheads in (A) and B mark glutamatergic and inhibitory CaMKIIa-RNs, respectively. C, D Heatmaps showing density of glutamatergic (Vglut2) (C) and inhibitory (Vgat) (D) CaMKIIa-RNs in MdV-IRt area. E Percentage of Vglut2 and Vgat positive CaMKIIa-RNs in MdV-IRt area. For identification of Vglut2 CaMKIIa-RNs and Vgat CaMKIIa-RNs: N = 3, n = 6 sections, 717 neurons, and N = 3, n = 6 sections, 287 neurons, respectively.
