Table 1 Comparison with pH50 values of previous studies

From: Molecular mechanisms and hotspots of pH sensing in ASIC1a revealed by computational and functional analysis

	This study		Interpretation other studies	Paukert^a		Kellenberger lab		Other studies
Residue	Activation	SSD		Mut	ΔpH50	Mut	ΔpH50	Mut	ΔpH50
Glu63			Eff.	Q**	0.10	C-AF488^e	0.40
His70						C-AF488^e	0.26
His72			Eff.	A	0.03	C-AF488^e	0.45
His73	Conf.		Eff.	A	−0.68			Cⁱ; A^o(ASIC2a)	−1.33; LOF
Lys76		Put.	Eff.			C**^h	−0.04	C^p	−1.44
Asp78	Eff.	Put.	Eff.	N**	−0.33	C**^h; C-AF488^c	−0.23, −0.21	A^o(ASIC2a)	LOF
Glu79	Eff.	Eff.	Eff.	Q	−0.11	C**^h, A^g	−0.14; −0.72	Q**^k; Q^l; R^o(ASIC2a)	−0.3; −0.8; LOF
Glu97		Eff.	Eff.			Q ^d; A^g	0.16; 0.1
His110			Eff.			C-AF488^c	0.04	A^o(ASIC2a)	LOF
His173		Put.						A^p	+0.07
Lys193			ND
Lys211	Conf.		Eff.	M**	−1			M^p, Eⁱ	−0.8, −0.77
Glu219	Put.		Eff.	Q	−0.1	A^g	−0.35	K^k	0.05
Glu238	Eff.	Put.	Eff.	Q	0	A^g	−0.33	Q^p, K**^k	−0.2, 0.1
Glu242	Conf.	Conf.	Eff.	Q	−0.12	A^g	−0.34
Lys246	Eff.	Eff.	Eff.	M	−0.16
Glu254		Put.		Q	−0.07	Q**^d	0.07
Asp259		Put.						Aⁿ	−0.21
Glu277	Eff.		Eff.			Q^d	−0.16
Lys291			ND
Asp298				N	0.08
Glu315	Put.	Eff.	Eff.	Q	0.12	Q**^d	−0.06
His329	Put.	Put.	Eff.	N	−0.48
Lys343				M	0.01			AzF^j	−0.25
Asp347	Eff.	Eff.	Eff.			N**^d, A^g	−0.19; −0.78	N**^k, N^m, N^l, N^p	0.12, −0.3, −0.29, −0.4
Asp351	Put.	Put.	Eff.			A^g	−0.32	N^k, N^m, N^l; AzF^j	0.28, 0.04, −0.17, −0.46
Glu355			Eff.			Q**^d, C-AF488^ce, A^g, Q^f	0.08; 0.4, 0.3; −0.18; 0.04	AzF^j	−0.25
Asp357	Eff.	Eff.	Eff.	N**	−0.01			AzF^j	−1.02
Glu364			ND
Lys374	Eff.	Eff.	Eff.	M	−0.23			AzF^j	−0.5
Glu375	Conf.	Conf.	Eff.	Q	−0.22	A^g	−0.22	AzF^j
Lys384		Put.	Eff.	M	−0.3
Lys388		Put.	Eff.	M	−0.46	C-AF488^ce	0.18, 0.32
Asp409		Eff.	Eff.	N	0.21	A^g	−0.18	K^k	0.17
Glu413	Conf.	Conf.		N	−0.09	Q**^d, A^g	−0.1; −0.24
Glu418	Conf.	Put.	Eff.	Q	−0.13	Q**^d, A^g	−0.2; −0.69	K^k, Q^l	−0.15; −0.81
Asp434			Eff.	C ^b	−0.23
Asp455		Put.	Eff.					AzF^j	−0.6

ΔpH50 = pH50(Mutant)-pH50(WT); “This study”: Put. putative pH sensor; Eff. substantial effect (= “functionally important residue”); Conf. confirmed pH sensor; (Eff. and Conf., according to criteria summarized in the discussion). Other columns; **, mutation to several residues tested; ND, not determined; C-AF488, Cys mutation, modified with fluorophore AF488; AzF, crosslinker⁷⁸; LOF, loss of function (in ASIC2a); the upper case single letters indicate residues (single letter code) by which the WT residue was replaced. In “Other studies, “Eff.” indicates that the pH50 shift was ≥ 0.15 of a conservative mutation, or ≥0.3 of a non-conservative mutation in ASIC1a.
References: a¹⁷,; b⁷⁹,; c⁴⁸,; d²⁰,; e⁵¹,; f⁴⁷,; g⁴³,; h⁸⁰,; i³³,; j⁷⁸,; k¹⁸,; l⁸¹,; m¹⁶,; n⁴⁶,; o²³,; p¹⁹,

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Table 1 Comparison with pH50 values of previous studies

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