Fig. 3: ATM inhibition decreases the knock-in efficiency with circular donor DNA but increases it with linear donor DNA. | Communications Biology

Fig. 3: ATM inhibition decreases the knock-in efficiency with circular donor DNA but increases it with linear donor DNA.

From: ATM Inhibition Enhances Knock-in Efficiency by Suppressing AAV-Induced Activation of Apoptotic Pathways

Fig. 3

a Genome editing results using a plasmid donor vector following treatment with two ATM inhibitors (10 nM AZD1390 and 1 μM AZ31). ATM inhibition significantly decreased the knock-in and EJ-TI efficiencies. Data are presented as mean ± SD. (n = 3 independent replicates). Dunnett’s multiple comparison test was conducted. b Genome editing results using an AAV donor vector following treatment with two ATM inhibitors (10 nM AZD1390 and 1 μM AZ31). ATM inhibition significantly increased the efficiencies of both knock-in and EJ-TI. Data are presented as mean ± SD. (n = 3 independent replicates). Dunnett’s multiple comparison tests were conducted. c Genome editing results using AAV donor vector following treatment with ATM inhibitor (10 nM AZD1390) in HEK293T cells and mouse MSC. In contrast to the triple-reporter ES cells, these experiments were performed using AAV donors designed to fuse EGFP to the C-terminus of the endogenous ACTB: only cells with a successful knock-in at ACTB express EGFP. Data are presented as mean ± SD. (n = 3 independent replicates). Unpaired t-tests were conducted.

Back to article page