Fig. 7: ATM inhibition reduces DNA-PK activity, impairing DSB repair via c-NHEJ.

a Western blotting results showing that treatment with the ATM inhibitor suppressed AAV-induced activation of DNA-PKcs, leading to reduced DSB repair via c-NHEJ. Protein was harvested 2 h after genome editing. b Quantification of Western blot results. The signal intensities were normalized to GAPDH. Fold changes are shown relative to the negative control. Data are presented as the mean ± SD. (n = 3 independent replicates). Tukey’s multiple comparison test was conducted.