Table 1 Determination of the PEO surface coverage of the AuNPs
From: Direct quantification of nanoparticle surface hydrophobicity
AuNP sample | A | B | C | D | E | F | G | H | I | L |
|---|---|---|---|---|---|---|---|---|---|---|
Volume of added PEO (µl) | 0 | 0.16 | 0.32 | 0.48 | 0.72 | 1.04 | 1.6 | 3.2 | 4.8 | 7.2 |
UV-vis peak spectral position (nm) | 543.3 | 546.2 | 546.4 | 546.4 | – | 546.6 | 546.7 | – | – | 546.9 |
DLS hydrodynamic diameter (nm) | 62.7 ± 10 | – | – | 63.1 ± 10 | – | 63.5 ± 10 | 64.9 ± 12 | – | – | 68.7 ± 15 |
Z-potential (mV) | −45 ± 2 | −42 ± 2 | −40 ± 2 | −41 ± 2 | – | −40 ± 2 | −36 ± 2 | – | – | −39 ± 2 |
DCS peak of the runtime (s) | 9.71 | 9.94 | 9.91 | - | 9.99 | 10.08 | 10.18 | 10.41 | 10.51 | 10.62 |
Number of PEO molecules per AuNP (1E4) | 0 | 5 | 16 | 20 | 18 | – | 55 | 194 | 210 | |
W/O partition, % of NPs in water | 0 | 25 | 38 | 36 | 68 | 77 | 92 | – | – | 100 |
SDS-PAGE, intensity of 40–200 KDa band (relative to pure HSA) (%) | 7.17 | – | – | – | 4.77 | 1.34 | – | 0.05 | 0.02 | 0 |
PEO surface coverage (theoretical) (%) | 0 | 5 | 10 | 15 | 22.5 | 32.5 | 50 | 100 | ≥100 | ≥100 |
