Fig. 5
From: On-cell catalysis by surface engineering of live cells with an artificial metalloenzyme
On-cell catalysis with preserved cell viability. Functionalization conditions: 2 (0.65 mM), 1 h, PBS; cofactor 9 (2.5 µM), streptavidin S112Y-K121R (10 µM free biotin-binding sites). a Uncaging of the proCoumarin 4 (400 µM, Kuhl medium) by the functionalized cells. Activity is given as fluorescence intensity coming from 6 in arbitrary units after 15 h at 25 °C. b Cell viability after reaction determined by flow cytometry with propidium iodide. a, b Data are represented as mean ± SD (n = 3) and analyzed by one-way ANOVA (post hoc Tukey test; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001). (1) Wild-type streptavidin was used in this case
