Fig. 4

The kinetics of strand displacement reactions. a Depiction of strand displacement reactions of TS-DS (P1a-P2b) with IS (P3a, P3c, and P3d). b Fluorescent measurements of strand displacement reactions as a function of time. c Result showing that a DNA invading strand (D3) was unable to displace P2b from P1a. d Comparison of strand displacement reactions between the conformationally-matched (green and red traces) and mismatched (yellow and blue traces) IS with TS-DS. e The rate constants of strand displacement reactions as shown in b and d. The concentrations of TS-DS duplexes were 100 nM and those of IS were 1 µM (10-fold excess). The samples were prepared in a physiologically relevant buffer, and the reactions were carried out at 37 °C. Excitation was made 344 nm, and the emissions were recorded at 480 nm