Fig. 3 | Communications Chemistry

Fig. 3

From: Stereochemical inversion as a route to improved biophysical properties of therapeutic peptides exemplified by glucagon

Fig. 3

Assessment of in vivo bioactivity. Lean male Sprague-Dawley rats received an intraperitoneal bolus of native glucagon (4) and d-amino acid analogs 16, 20 and 21 and blood glucose was monitored for 120 min. Peptides were formulated in 50 mM Tris buffer pH 8 at 50 μM concentration; and dosed at 30 nmol/kg. a Change in blood glucose relative to Time = 0 min. b Calculated area under the curve of the blood glucose change from 0–60 min. Data are expressed as mean ± standard error, n = 6. ****P < 0.0001, one-way ANOVA, followed by Dunnett’s tests, using the vehicle group as control

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