Table 1 4,6-Disubstituted-N-heterocycles by (tandem) CMPS catalysis

From: Biocatalytic production of bicyclic β-lactams with three contiguous chiral centres using engineered crotonases

  1. See Fig. 3 for reaction scheme
  2. aThe C-2-alkylated-malonyl-CoA derivatives are Ccr or MatB products (see text and Fig. 6 for details)
  3. bd.r.: diastereomeric ratio of epimers at C-4 and/or C-6 of t-CMP derivatives, determined by 1H NMR and/or LC–MS, under standard conditions. cThe % yield (isolated) was calculated10,11,25 following deprotection of amino acid aldehydes, incubation with enzyme(s), LC–MS purification and lyophilisation; products were quantified by NMR using [2H]4-trimethylsilylpropionate as a standard
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