Fig. 6
From: Hierarchical self-assembly and emergent function of densely glycosylated peptide nanofibers
GQ11 gels selectively recognized a GlcNAc-binding protein but otherwise resisted non-specific biological interactions. a Bovine serum albumin rapidly released from NQ11 and GQ11 hydrogels, whereas b wheat germ agglutinin was selectively retained by GQ11 hydrogels. Dashed lines in a and b represent mass of protein encapsulated within the gel. GQ11 hydrogels were more resistant to non-specific RAW264.7 macrophage adhesion than NQ11 hydrogels in the presence of serum as determined c quantitatively via metabolic activity and d, e qualitatively via light microscopy. f–h GQ11 hydrogels were also more resistant to non-specific NIH3T3 fibroblast adhesion in the presence of serum than NQ11 hydrogels. i Glass surfaces coated with GQ11 hydrogels resisted non-specific NIH3T3 fibroblast adhesion. j Region of GQ11 hydrogel represented by white box in i. In contrast, k regions of glass coated with NQ11 hydrogels were fouled by NIH3T3 fibroblasts. l Region of NQ11 hydrogel represented by white box in k. m GQ11 hydrogels were also more resistant to E. coli adhesion than NQ11 hydrogels. In i–l, nanofibers were stained green with Thioflavin T (ThT) and cells were stained red with CellTracker®. Scale bar = 100 μm in d, e, g–i, and k. Data presented as mean ± standard deviation in a–c, f, and m. **p < 0.005, and ***p < 0.0005 using Student’s t test (n = 3)
