Fig. 4: Structure determination of the basic building blocks of the SLP fibers. | Communications Chemistry

Fig. 4: Structure determination of the basic building blocks of the SLP fibers.

From: Control over the fibrillization yield by varying the oligomeric nucleation propensities of self-assembling peptides

Fig. 4

a Superposition of 2D ssNMR PARIS 13C–13C spectra of 13C,15N-(Val1, Val2, Leu6, Leu7)-labeled SLP1 acquired with 50 (gray) and 700 ms (magenta) magnetization transfer. Intramolecular and intermolecular correlations are labeled in black and blue, respectively. The spectrum with 700 ms magnetization transfer time was acquired with a CPMAS CryoProbe (Bruker Biospin). b Inter-residual magnetization transfer from Val1/Val2 to Leu6/Leu7 observed in the 2D 13C–13C ssNMR spectrum relates to intermolecular contacts between antiparallel β-strands. Distances are Cα–Cα spacings. c ssNMR CαCβ secondary chemical shifts show that the hydrophobic tails adopt β-strand conformation in the SLP1 nanofiber, which is similar for SLP2 and SLP3 fibers60. d ssNMR structure of the SLP1 dimer in the 1D nanofiber. A superposition of the three best structures is shown (backbone RMSD of 1.11 Å). Oxygen atoms of the anionic C-termini are highlighted as red spheres.

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