Fig. 5: Spatiotemporally resolved fluorescence labeling of live cells. | Communications Chemistry

Fig. 5: Spatiotemporally resolved fluorescence labeling of live cells.

From: Direct ring-strain loading for visible-light accelerated bioorthogonal ligation via diarylsydnone-dibenzo[b,f ][1,4,5]thiadiazepine photo-click reactions

Fig. 5

a Schematic illustration of photo-click processes on live cells via 405 nm light induced DASyd-DBTD ligation toward Cetuximab-DBTD (Cex-DBTD). b Colocalization imaging experiments of Cyanine-3 (Cy3), fluorescein-5-isothiocyanate (FITC) and NucBlueTM fluorescence channels with control groups. c Spatial control of fluorescent labeling on cell membrane surface with irradiation of 390 nm light. d SDS-PAGE analysis of the cell lysates of Cex-DBTD bound A549 cells with or without irradiation. M. = protein size marker.

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