Fig. 2: FIH can catalyse dihydroxylation reactions.
a FIH catalyses single- and double-hydroxylations of (L)- and (D)-leucine-containing residues, respectively. b Mass spectrometric analysis of FIH-catalysed hydroxylation of a (D)-leucine residue in a consensus ankyrin (CA) (HLEVVKLLLEHGADV((D)-L)AQDK) substrate. The CA (D)-Leu peptide (50āĪ¼M) was incubated with 0āĪ¼M, 25āĪ¼M, 50āĪ¼M and 100āĪ¼M FIH. Sub-stoichiometric quantities of FIH result in predominantly mono-hydroxylated product, whereas at higher FIH concentrations the dihydroxylated product is produced. Note the different C3 hydroxylation stereochemistries for the (L)-Leu and (D)-Leu substrates. See text for discussion.
