Table 4 Guide for using the 2-ethynylbenzaldehydes for N-terminal selective modification.
From: N-Terminal selective modification of peptides and proteins using 2-ethynylbenzaldehydes
Reaction conditions | Peptides | Proteins |
|---|---|---|
Equivalent of 2-EBA | 20 equivalents | ≤10 equivalents |
pH | 6.5 | 6.5–7.4 |
Reaction temperature | 37 °C | |
Reaction time | ≤16 h | |
Substituent effects of 2-EBA | N-terminal selectivity is excellent (up to >99:1) when 2-EBA with electron-donating group at 5- or 4-positions. | |
Functional group tolerance | Highly compatible with 19 of the 20 natural amino acids, except for N-terminal proline. | |
