Fig. 4: GMP labeling of SARS-CoV-2 nsp7 mutants.
From: Mass spectrometric based detection of protein nucleotidylation in the RNA polymerase of SARS-CoV-2
a Radiolabeling of nsp7 was assessed after mutating indicated residues to alanine. A Coomassie-stained SDS-PAGE gel and an autoradiograph are shown. b Quantitation of mutant radiolabeling relative to WT in two separate experiments normalized to nsp8 signal. Individual datapoints (black diamonds) are shown (n = 2). Data are scaled to WT signal that was set to 100 percent, as indicated by the dotted line. c LC–MS precursor ion quantification of percent (%) GMP modification of the SARS-CoV-2 peptide 1–14 upon introduction of indicated mutations in comparison to total peptide amount (unmodified and modified). Each bar represents a single data point. Two experiments were performed for WT and for the S2A, K3A mutation. A single experiment was run for the nsp7 S2A and nsp7 K3A mutant proteins.
