Fig. 1: Diagram of ADP probes and their biophysical characterization.
a Structure of ADP-1 and ADP-2 probes and the scheme of their photo-liberating reaction. b Time-course transmission electron microscope analysis of ADP-1 (50 μM). Scale bars indicate 200 nm. c Dot blot analysis of ADP-1-induced GA DPRs over time. The sample was incubated until the indicated time and applied on the membrane. Membranes were stained with anti-GA DPRs antibody and A11 antibody. N = 3. d Time-course turbidity measurements of irradiated (wavelength: 365 nm, power density: 32 mW/cm2, duration: 1 min) (red) and unirradiated (black) ADP-1 (50 μM). Data are collected at 0.5th, 2nd, 6th, 12th, 24th, 48th, and 96th hour (Standard deviation, N = 3). e Fluorescence lifetime images and the histograms of the photoinitiated ADP-2 (5 μM). Images were taken at the 0.5th, 2nd, and 24th hours of incubation; scale bars indicate 5 µm. f Epi-fluorescence and direct stochastic optical reconstruction microscopy (dSTORM) images of the photoinitiated ADP-2 (50 μM). Images were acquired at the 12th and 24th hours of incubation; scale bars indicate 10 µm.
