Fig. 2: GA DPRs delivered by ADP-1 and 2 probes oligomerized in cells.
a Time-course fluorescence lifetime images and histograms of ADP-2 in SH-SY5Y cells. 1 μM ADP-2 were treated to cells and photoinitiated by UV light (wavelength: 335–379 nm, power density: ≤8.24 mW/cm2, duration: 1 min). Cell periphery was contoured with a white line in 12th and 24th hours, respectively. Images were taken at 2nd, 12th, 24th hour after UV illumination; scale bars indicate 5 µm. b A11 immunoblotting with cell lysates from SH-SY5Y cells received ADP-1 (1 µM) treatment with or without photoinitiation. After photoinitiation, cell lysates at different time points were harvested and analyzed. c Quantification analysis of A11 immunoblot of SH-SY5Y total cell lysates. The signal of A11 staining was first normalized to GAPDH staining and then compared to signal at 0.5 h incubation. Three biological replicates were carried out (r = 3). Mean and standard deviation for ADP-1, UV = 171.7 ± 17.0; ADP-1, dark = 85.8 ± 10.6. ** indicates statistical significance where p value < 0.01 (analyzed by two-sided Welch’s T test, p value = 0.005, degree of freedom = 3, t value = 3.18).
