Fig. 2: RquA kinetic study. | Communications Chemistry

Fig. 2: RquA kinetic study.

From: Microbial rhodoquinone biosynthesis proceeds via an atypical RquA-catalyzed amino transfer from S-adenosyl-L-methionine to ubiquinone

Fig. 2

a Time course chromatograms measuring the production of rhodoquinone-3 (RQ3) are overlaid for an MBP-RquA in vitro assay monitored by liquid chromatography-mass spectrometry (gray: 0 min, magenta: 4 min, green: 12 min, orange: 20 min, purple: 28 min, blue: 68 min) after ubiquinone-3 (UQ3) (1 μM) was added to MBP-RquA (0.5 μM) with S-adenosyl-L-methionine (SAM, 5 μM). b A Michaelis–Menten curve was generated for varying UQ3 concentrations (0.05–1 µM) at 0, 4, 8, and 16 min time points with MBP-RquA (0.5 μM) and SAM (5 μM). c A second Michaelis–Menten curve was generated for varying SAM concentrations (0.1–2.5 µM) at the same time points with MBP-RquA (0.5 μM) and UQ3 (5 μM). The Vmax corresponds to a peak specific activity of 0.22 pmol min−1 μg protein−1. For b, c, n = 3 independent experiments and error bars represent the standard deviation of the mean.

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