Fig. 3: Expression of GFP-RquA in S. cerevisiae. | Communications Chemistry

Fig. 3: Expression of GFP-RquA in S. cerevisiae.

From: Microbial rhodoquinone biosynthesis proceeds via an atypical RquA-catalyzed amino transfer from S-adenosyl-L-methionine to ubiquinone

Fig. 3

a Confocal microscopy images of yeast expressing GFP-RquA were imaged at 405 nm (DAPI: red) and 510 nm (GFP: cyan). The merged image (top right) shows an overlap of the GFP with the DAPI, indicating the localization of RquA in the mitochondria. b Yeast harboring pRCM_GFPRquA produce rhodoquinone-6 (RQ6, dark blue), and supplementation with 15N-L-methionine yields 15N-RQ6 (light blue), The production of ubiquinone-6 (UQ6) is shown in red. n = 2 biologically independent samples and error bars represent the standard deviation of the mean. c The experimental mass of synthesized [15N-RQ6 + H]+ was determined to be 577.4373 m/z (C38H5815NO3 exact mass = 577.4387 m/z, mass accuracy = −2.4 ppm).

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