Fig. 2: Optimizations of the strain and bioprocess. | Communications Chemistry

Fig. 2: Optimizations of the strain and bioprocess.

From: De novo biosynthesis of berberine and halogenated benzylisoquinoline alkaloids in Saccharomyces cerevisiae

Fig. 2

Production of (a) berberine, and (b) canadine, in strain BBR3. Extracted ion chromatogram (EIC) of berberine (m/z 336.123) and canadine (m/z 340.154) are shown. Traces are representative of three biological replicates. Titer of (c) berberine, and (d) canadine, in optimized strain BBR4R compared with original BBR3R. Two-tailed t-test was used to calculate the p-value: *p < 0.05 and **p < 0.01. e Screening of active STOX in N. benthamiana transient expression system with canadine infiltrated. f Increased concentration of berberine and possible intermediates under heating condition. Independent experiments, n = 3. Error bars represent standard deviation.

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