Fig. 4: Production of F-substituted BIAs in BBR4R from 3-F-tyrosine feeding. | Communications Chemistry

Fig. 4: Production of F-substituted BIAs in BBR4R from 3-F-tyrosine feeding.

From: De novo biosynthesis of berberine and halogenated benzylisoquinoline alkaloids in Saccharomyces cerevisiae

Fig. 4

Schema of proposed semi-biosynthetic pathway of F-BIAs from 3-F-tyrosine. Dopamine route (blue line) and 4HPAA route (green line) are shown. The high-resolution EIC of (a) 8, 3’-di-F-Coclaurine, (b) i) 8-F-Coclaurine, ii) 3’-F-Coclaurine, (c) 3’-F-NMC, (d) 3’-F-Reticuline, (e) 11-F-Scoulerine, (F) 11-F-THCB. The EIC was extracted using the calculated m/z of target compounds (M + H)+, with a mass accuracy below 100 ppm. Traces are representative of three biological replicates.

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