Fig. 2: Scheme of the PEX construction of modified DNA and denaturing PAGE analysis of PEX reaction with DNA random library using KOD XL DNA polymerase. | Communications Chemistry

Fig. 2: Scheme of the PEX construction of modified DNA and denaturing PAGE analysis of PEX reaction with DNA random library using KOD XL DNA polymerase.

From: The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70

Fig. 2

Scheme of the PEX (A); Denaturing PAGE analysis (B): Lane 1 (P): primer, Lane 2 (Nat): DNA library amplified by natural dNTPs, Lane 3 (Mod): DNA library amplified with modified dA*TP and natural dCTP, dGTP, dTTP, Lane 4 (Pool): DNA library scaled-up for generation of modified starting library for SELEX, Lane 5 (L): FAM-labelled ssDNA ladder.

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