Fig. 5: Binding affinity results of the natural HSNat-9 sequence, modified HSc-9 aptamer and the modified truncated HSc-9.1 aptamer. | Communications Chemistry

Fig. 5: Binding affinity results of the natural HSNat-9 sequence, modified HSc-9 aptamer and the modified truncated HSc-9.1 aptamer.

From: The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70

Fig. 5

a–c The potential predicted secondary structures for the HS-9 aptamers as determined by MFold. PhBu-modifed dA* bases with side chains are highlighted in red for visualisation. d–f The corresponding binding curves for HS-9 aptamers as determined by BLI, different aptamer concentrations were used as analyte and the steady state binding affinity [Kd] was calculated. g–i The corresponding binding curves for HS-9 aptamers as determined by the fluorescent-plate-based binding assay. The raw fluorescent data were exported into Graph Prism and the binding affinity [Kd] of each aptamer calulated. Error bars represent the average values of two independent experiments.

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