Table 2 Selection conditions used in each round of the modified SELEX procedure against Hsp70.

From: The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70

SELEX round

ssDNA (nM)

Hsp70

(µg)

Time (min)

Competitors

(1 mg/mL BSA + 0.1 mg/mL salmon sperm DNA)

Washing conditions

R1[a]

500

10

50

-

3 × 1 mL for 1 min

R2[a]

100

10

50

-

3 × 1 mL for 1 min

R3[a]

70

10

40

-

3 × 1 mL for 1 min

R4[a]

70

5

40

Yes

3 × 1 mL for 1 min

R5[a]

70

4

30

Yes

3 × 1 mL for 2 min

R6[a]

50

3

30

Yes

3 × 1 mL for 2 min

R7[b]

15

3

30

Yes

5 × 1 mL for 2 min

R8[b]

15

2

30

Yes

5 × 1 mL for 5 min

R9[b]

15

1

30

Yes

5 × 1 mL for 5 min

  1. Incubation carried out in 250 µL and at 24 °C. [a] Negative selection performed against the beads only before incubation with the target. [b] Negative selection with His-NPA before incubation with the target.
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