Table 3 List of the most abundant aptamer sequences obtained after SELEX against Hsp70.

From: The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70

Apt ID

Sequence 5′→ 3′ with primer region underlined

NGS

(%)

HSe-1[a, b]

GCAGCAGAGATAGACGCTATTTCTTA*GA*CCTTTCTA*A*TTTA*A*CTA*CTCA*A*TGGCA*TA*GCTGGCA*C

8.37

HSe-2[a, b]

GCAGCAGAGATAGACGCTATTCTCTA*GA*CCTTCTA*A*TTTA*CA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C

4.57

HSe-3[a, b]

GCAGCAGAGATAGACGCTAA*GGGA*A*GTA*GGA*A*GA*A*GGGA*TGCCCTGCA*A*TGGCA*TA*GCTGGCA*C

1.58

HSe-4[a, b]

GCAGCAGAGATAGACGCTAGGA*TCTCA*TA*GTTCTTGTA*A*TGA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C

1.47

HSe-5[a, b]

GCAGCAGAGATAGACGCTAA*TCGA*TGA*GA*CCTTCTA*A*TTA*A*CTA*TTCCA*A*TGGCA*TA*GCTGGCA*C

0.78

HSe-6[a, b]

GCAGCAGAGATAGACGCTATCTCTTA*GA*CCTTCTA*A*TTTA*A*CTA*CTCA*A*TGGCA*TA*GCTGGCA*C

0.74

HSe-7[a, b]

GCAGCAGAGATAGACGCTAGA*TCTCCA*GTCCTTCTA*A*TTA*A*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C

0.43

HSe-8[a, b]

GCAGCAGAGATAGACGCTAGTCCTCA*TA*GGCCTTCTA*A*TTA*A*CTA*TTCA*A*TGGCA*TA*GCTGGCA*C

0.21

HSe-9[a,b]

GCAGCAGAGATAGACGCTAA*GA*CCTCA*TA*GTCCTTCTA*A*TTA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C

0.12

HSc-2[c]

GCAGCAGAGATAGACGCTATTCTCTA*GA*CCTTCTA*A*TTTA*CA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C

 

HSc-9[c]

GCAGCAGAGATAGACGCTAA*GA*CCTCA*TA*GTCCTTCTA*A*TTA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C

 

HSc-9.1[c]

A*GA*CCTCA*TA*GTCCTTCTA*A*TTA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C

 
  1. [a] Reverse complement sequence purchased 5′-biotinylated; [b] 5′-6-FAM-labelled reverse primer used for PEX production of dA*-modified strand; [c] aptamers synthesised both with and without 5′-6-FAM label; HSe enzymatically synthesised; HSc chemically synthesised.