Table 1 Kinetic and thermodynamic parameters of protein metamorphism in dilute buffer and crowded conditions.
From: Crowded environments tune the fold-switching in metamorphic proteins
| Â | KaiBG89A | |||||
|---|---|---|---|---|---|---|
kex(h-1) | Pgs | Pfs | k1(h-1) | k-1(h-1) | ΔG(kJ/mol) | |
Buffer | 0.44 ± 0.02 | 0.80 ± 0.01 | 0.2 ± 0.01 | 0.09 ± 0.01 | 0.35 ± 0.01 | 3.49 ± 0.16 |
Ficoll400 | 0.42 ± 0.01 | 0.85 ± 0.02 | 0.15 ± 0.02 | 0.06 ± 0.01 | 0.36 ± 0.02 | 4.37 ± 0.34 |
BSA | 0.48 ± 0.05 | 0.93 ± 0.01 | 0.07 ± 0.01 | 0.03 ± 0.01 | 0.45 ± 0.04 | 6.52 ± 0.51 |
| Â | KaiBD91R | |||||
Buffer | 0.28 ± 0.01 | 0.64 ± 0.01 | 0.36 ± 0.01 | 0.10 ± 0.01 | 0.18 ± 0.01 | 1.45 ± 0.05 |
Ficoll400 | 0.28 ± 0.01 | 0.64 ± 0.01 | 0.36 ± 0.01 | 0.10 ± 0.01 | 0.18 ± 0.01 | 1.45 ± 0.03 |
BSA | 0.24 ± 0.01 | 0.71 ± 0.01 | 0.29 ± 0.01 | 0.07 ± 0.01 | 0.17 ± 0.01 | 2.26 ± 0.07 |
| Â | XCL1 | |||||
Buffer | 4.81 ± 0.04 | 0.37 ± 0.001 | 0.63 ± 0.001 | 3.03 ± 0.02 | 1.78 ± 0.01 | −1.39 ± 0.006 |
Ficoll400 | 4.30 ± 0.38 | 0.44 ± 0.001 | 0.56 ± 0.001 | 2.41 ± 0.18 | 1.89 ± 0.20 | −0.63 ± 0.006 |
PEG10K | 2.08 ± 0.32 | 0.58 ± 0.04 | 0.42 ± 0.04 | 0.87 ± 0.09 | 1.21 ± 0.23 | 0.84 ± 0.04 |
