Fig. 3: Melting temperature of WT and UGT variants and the key stabilizing mutations.
From: Enhancing the thermostability and activity of glycosyltransferase UGT76G1 via computational design
a Melting temperatures (Tm) of the wild-type (WT) and five designed variants. Variant 76_4 is abbreviated as V4 (cyan), 76_5 as V5 (bluish green), 76_6 as V6 (blue), 76_7 as V7 (red), and 76_8 as V8 (brown). b A comparison of the change in Tm (ΔTm), the absolute value of ΔΔG (represented by a circle with a cross), and the number of mutations (indicated by a black star). c The key mutations that potentially impact the thermostability of the variants. Noncovalent interactions are represented by dashed lines, with hydrogen bonds shown in yellow and π–π or cation–π interactions displayed in teal. The mutations present in each variant are indicated above their respective small boxes.
