Fig. 4: In vitro binding assay and cytotoxicity study.

a B-lymphoma cells were treated with NK20a, and the fluorescence of the treated cells was analyzed through flow cytometry to determine the binding ability of NK20a to the cell surface. The fluorescence distribution of FITC was categorized as treated with NK20a (green) and untreated (red). b Bar chart showing the cell viability of the B-lymphoma cell line treated with 10 ng/mL IL-10, 50 μg/mL NK20a, and both, respectively. Error bars represent the standard deviation of experiments done in triplicate.