Table 1 Large-scale and structurally controllable synthesis of gangliosides with different glycosyl groups .

From: A modular chemoenzymatic cascade strategy for the structure-customized assembly of ganglioside analogs

  1. Condition a: Substrates 11–15, substrate 16 (2.40 g, 8.00 mmol), and purified EGC-II E351S/D314Y (800 mg) were incubated in 100 mM NaAc buffer (pH 5.3) with 10% ethanol (v/v) at 37 °C for 10 h. Yield after purification using C18 cartridges is shown.
  2. Condition b: Substrates 6–10 (2.00 mmol), stearic acid (853 mg, 3.00 mmol), and purified SA_SCD (160 mg) were incubated in 800 mL of Tris-HCl buffer (50 mM, pH 7.5) with 10% DME (v/v) at 37 °C for 6 h. Yield after purification using Sep Pak VAC C18 cartridges and silica gel filtration column is shown. Abbreviations: EGC-II E351S/D314Y, endoglycoceramidase II E351S/D314Y. SA_SCD, Shewanella alga G8 sphingolipid ceramide N-deacylase (see more details in Supplementary II).
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